Sunday, May 17, 2020

Summary Of A Christmas Carol - 11802 Words

A Christmas Carol Setting There are multiple locations and time periods to this play.There is no set country so no accents are needed. The narration takes place in a living room during a storm and a black out in the modern times( 2000-now). Scrooges’ story takes place in the 1920’s (depression era),The Spirits bring him back to 1900’s,and the â€Å"future† maybe 5 years ahead from the main story. Characters 6M/4F/10either doubling option/Extras optional There are 3 Narrators in the present( 2000-now) Grandpa†¦..Approx 70-80 years old. Still has pep in his step and a young misdemeanor 32 Grandma†¦..Same age as Grandpa. Young at heart 21 Grandchild...Boy or girl can be Robert or Roberta 27 Story within the story 1920’s Child 1†¦Ã¢â‚¬ ¦.Boy or girl dressed for outdoors in winter 4 Child 2†¦Ã¢â‚¬ ¦..same 3 Multiple children can be added as wanted. can be a large group or 2-3 Scrooge†¦..Hard,unfeeling,no nonsense, business minded,makes a dramatic transformation Bob Cratchit..Kindly man, father,husband,easy going Fred†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.. Scrooges’ nephew,only child of his beloved sister. Happy,hopeful,fun loving( can be played as a niece with a name change) 3 Collectors.. Collecting for the poor either male or female Marley†¦Ã¢â‚¬ ¦.. Scrooge’s old partner, appears as a ghost in chains with money boxes Spirit of Christmas past†¦.male or female..ethereal beauty Young Scrooge:....Still had hope and love of man kind Fan:.....................Scrooges beloved sister of ScroogeShow MoreRelatedScrooge Is A Miserable And Greedy Old Man Essay1390 Words   |  6 PagesEbenezer Scrooge is a miserable and greedy old man, who focuses mainly on his business and continues with his greedy ways on Christmas Eve. He is always grumpy and yells at his employees, and most of all at Bob Cratchit. Scrooge’s desire is to be by himself on Christmas Eve, and rejects his nephew Fred’s, invitation for dinner. One night, the ghost of Jacob Marley, a former colleague of Scrooge, who has passed away seven years ago, visits the old man. Marley reminds Scrooge of his cruel lifestyleRead MoreDrama And Themes Of Drama1111 Words   |  5 Pagescomedy, action, romance, etc., in turn making it the most common genre of movies (Dirks, 2015). Drama focuses on realistic characters with a realistic story line, high intensity special effects are not usually prevalent in dramas. The Help, A Christmas Carol, Mr. Mom, ar e the dramas chosen that I feel depict race, social class, and gender representation each at a very different level, based on the time period implied in the movie. COMMONALITIES AND CHOSEN ISSUES If I am going to see a movie basedRead MoreGiovanni s Room By James Baldwin1723 Words   |  7 Pagescharacter Therese finds herself attracted to a mature lady named Carol. Therese is a young lady who works in retail selling dolls at a department store in New York City. She was an aspiring set designer, but her seasonal job for the winter was selling dolls that end up peeing themselves as gifts for young girls. Therese meets Carol because she was one of the customers that caught her attention trying to buy her daughter a doll for Christmas. After leaving, she ends up leaving her one of her belongingsRead MoreThe Music Of A Christmas Carol1333 Words   |  6 Pageswhen Hester claims that she thought it was her playing the organ (Gaskell 18). Being that Miss Furnivall is deaf, and is therefore an unlikely musician, this insinuates that Miss Furnivall is connected to this problem. However similar to â€Å"A Christmas Carol†, the music is suggested to be derived from ghostly m eans, because the organ is found to be broken inside, therefore unable to be played normally (Gaskell 18). This thought is later underlined when the child, Rosamond, goes missing, and ghostsRead MoreVictorian Ghost Stories Nearly Always Encompass Family1361 Words   |  6 Pagespresent throughout many of the periods ghost stories and other genres. The Victorians also created the modern Christmas, where families come together on Christmas Eve to read or re-tell popular Ghost stories of the time, many magazines which were published in the Christmas period contained ghost stories , with one of the most popular being Charles Dickens, A Christmas Carol, the Christmas period can be seen as the perfect time to introduce ghost stories are the days are shorter, meaning longer nightsRead MoreGoodnight Mr Tom Chapter Breakdown2051 Words   |  9 PagesChapter 2, Little Weirwold Summary This chapter begins with Mr. Tom continuing to explain the dog to Willie. The old man shows the boy how to make friends with the dog and how to help gain its trust. Mr. Tom begins the day by handing Willie a mug of hot, sweet tea, a preview of the many kindnesses that the old man will show to Willie, as a father to son would do. Willie gives up his fear of the dog and allows it to lick his hand, learning that the licking means the dog likes him. On the way outRead MoreDissociative Identity Disorder And Media Depictions3153 Words   |  13 Pagesfinds nine taped sessions of a patient by the name of Mary Hobbes, who had three different personalities (The Princess, Billy and Simon). In the tapes, the doctor who was treating Mary keeps asking her and her personalities about what happened on Christmas night in Lowell when she was a child. More and more details are uncovered in each session but one thing remains constant, neither Billy nor the Princess want to talk about Simon. The doctor continues to try and coax him out with no luck until theRead MoreThe Evidence Of Unbound Loyalty1746 Words   |  7 Pagespresents many captivating ideas, the focus of this particular monograph will concentrate on the unfailing loyalty of his characters and how this faithfulness will lead to their benefit or downfall. Before delving too deep in detail however, a brief summary must be presented. Oliver is born an orphan into a workhouse in a quaint little town about 70 miles outside of London, England. Oliver spends the first eight years of his life abused and neglected in a â€Å"child farm† ran by the parish, and soon afterRead MoreEssay Cry Baby Complaints2299 Words   |  10 PagesEXECUTIVE SUMMARY The Company had received a number of complaints about the product of â€Å"Cry Baby† doll after Christmas. The complaints were about the malfunction of the product that leakage of water and unable to cry when pressed the certain button, and the doll’s eyes were easily removed that harmful to children potentially. For that reason, an investigation into the incident was conducted. After interviewed with staff, the 120 complainants and looked into the data, reports and guidelines, weRead MoreRe Sba on Festivals in Christianity2193 Words   |  9 Pages 1 Information Collection 2 Summary of Findings 3 Conclusion

Wednesday, May 6, 2020

The Scarlet Letter By Nathaniel Hawthorne - 1533 Words

The Scarlet Letter by Nathaniel Hawthorne highlights the concepts of sympathy and shame through Arthur Dimmesdale, who commits a sinful act of adultery. Dimmesdale is a renowned minister in Puritan society who conceives an illegitimate child with Hester Prynne. Dimmesdale is not publicly condemned; instead, as he conceals his sin from public scrutiny, he faces an inner conflict. He is conflicted because if he confesses, he can become Hester’s lover, but will also be publicly scrutinized. On the other hand, if he continues to conceal his sin, he will continue feeling shameful, but can remain renown in the Puritan community. Before Dimmesdale dies, he overcomes his inner conflict and is able to atone for his wrong doings. Dimmesdale’s complex character and the particular circumstances of his crime, ultimately makes Hawthorne and readers ambivalent towards Dimmesdale’s plight (150). Dimmesdale is a renowned individual of Puritan society, so he is greatly influenced by the â€Å"iron framework of reasoning† (150). The Puritan settlement depicted in the novel is strict in their values. As a result, Dimmesdale is greatly ashamed of his transgression because adultery is one of the greatest crimes in Puritan society. Dimmesdale believes that it is his obligation to abide by Puritan principles, so he is repentant of his iniquity. When Hawthorne reflects on adultery, he says, â€Å"a penalty which, in our days, would infer a degree of mocking infamy and ridicule, might then be invested withShow MoreRelatedThe Scarlet Letter By Nathaniel Hawthorne1242 Words   |  5 PagesLYS PAUL Modern Literature Ms. Gordon The Scarlet Letter The scarlet letter is book written by Nathaniel Hawthorne who is known as one the most studied writers because of his use of allegory and symbolism. He was born on July 4, 1804 in the family of Nathaniel, his father, and Elizabeth Clark Hathorne his mother. Nathaniel added â€Å"W† to his name to distance himself from the side of the family. His father Nathaniel, was a sea captain, and died in 1808 with a yellow fever while at sea. That was aRead MoreThe Scarlet Letter By Nathaniel Hawthorne960 Words   |  4 Pages3H 13 August 2014 The novel, The Scarlet Letter, was written by the author Nathaniel Hawthorne and was published in 1850 (1). It is a story about the Puritan settlers of the Massachusetts Bay Colony, set around 1650 (2). The story is written in the third person with the narrator being the author. The common thread that runs through this novel is Hawthorne’s apparent understanding of the beliefs and culture of the Puritans in America at that time. 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The Puritans were in a religious period that was known for the strict social norms in which lead to the intolerance of different lifestyles. Nathaniel Hawthorne uses the puritan’s strict lifestyles to relate to the universal issues among us. The time frame of the puritans resulted in Hawthorne eventually thinkingRead MoreThe Scarlet Letter By Nathaniel Hawthorne999 Words   |  4 Pages Nathaniel Hawthorne is the author of the prodigious book entitled The Scarlet Letter. In The Scarlet Letter, Hester Prynne commits adultery with Reverend Arthur Dimmesdale. Her husband, Roger Chillingworth, soon finds out about the incident after it becomes clear that she is pregnant. The whole town finds out and Hester is tried and punished. Meanwhile, Roger Chillingworth goes out then on a mission to get revenge by becoming a doctor and misprescribing Dimmesdale. He does this to torture DimmesdaleRead MoreThe Scarlet Letter, by Nathaniel Hawthorne1037 Words   |  5 Pagesthat human nature knows right from wrong, but is naturally evil and that no man is entirely â€Å"good†. Nathaniel Hawthorne, author of the classic novel The Scarlet Letter, believes that every man is innately good and Hawthorne shows that everyone has a natural good side by Hester’s complex character, Chillingworth’s actions and Dimmesdale’s selfless personality. At the beginning of the Scarlet Letter Hester Prynne is labeled as the â€Å"bad guy†. The townspeople demand the other adulterer’s name, butRead MoreThe Scarlet Letter By Nathaniel Hawthorne1517 Words   |  7 PagesNathaniel Hawthorne composes Pearl as a powerful character even though she is not the main one. Her actions not only represent what she is as a person, but what other characters are and what their actions are. Hawthorne makes Pearl the character that helps readers understand what the other characters are. She fits perfectly into every scene she is mentioned in because of the way her identity and personality is. Pearl grows throughout the book, which in the end, help the readers better understandRead MoreThe Scarlet Letter, By Nathaniel Hawthorne1488 Words   |  6 Pages In Nathaniel Hawthorne’s novel The Scarlet Letter, the main character, Hester Prynne, is a true contemporary of the modern era, being cast into 17th century Puritan Boston, Massachusetts. The Scarlet Letter is a revolutionary novel by Nathaniel Hawthorne examining the ugliness, complexity, and strength of the human spirit and character that shares new ideas about independence and the struggles women faced in 17th century America. Throughout the novel, Hester’s refusal to remove the scarlet letterRead MoreThe Scarlet Letter By Nathaniel Hawthorne1319 Words   |  6 PagesPrynne and Arthur Dimmesdale are subject to this very notion in Nathaniel Hawthorne s The Scarlet Letter. Hester simply accepted that what she had done was wrong, whereas Dimmesdale, being a man of high regard, did not want to accept the reality of what he did. Similar to Hester and Dimmesdale, Roger Chillingworth allows his emotions to influence his life; however, his influence came as the result of hi s anger. Throughout the book, Hawthorne documents how Dimmesdale and Hester s different ways of dealingRead MoreThe Scarlet Letter By Nathaniel Hawthorne1714 Words   |  7 PagesSome two hundred years following the course of events in the infamous and rigid Puritan Massachusetts Colony in the 1600s, Nathaniel Hawthorne, descendant of a Puritan magistrate, in the 19th century, published The Scarlet Letter. Wherein such work, Hawthorne offered a social critique against 17th Massachusetts through the use of complex and dynamic characters and literary Romanticism to shed light on said society’s inherent contradiction to natural order and natural law. In his conclusive statements

The Exchange of the Relationship of the Ras function as a GTPase

Question: Explain Relationship of the Ras function as a GTPase? Answer: Introduction The ras p21 proteins belong to the family of the related polypeptides found in all the eukaryotic living organisms from yeast to human. Their exceptional developmental conservation recommends that they have the fundamental cell capacities, in spite of the fact that the specific role they play remains obscure. The mutations in particular amino acids and the overexpression of ordinary proteins have got connected to the changed proliferation as well as the differentiation and, especially, to neoplastic procedures. The mature ras p21 proteins happen to get situated on the internal side of the plasma layer(Rhodes, et al., 2013). Furthermore, their biochemical properties incorporate the binding and the exchange of the guanine nucleotides and the GTPase activity. The favored speculation for ras capacity is that these proteins exist in harmony between a dormant compliance (p21.GDP) and the active conformation (p21. GTP) in which they can interface with their up 'til now obscure cell target o r targets. Likenesses in the cell area, structure, and the biochemistry with other regulatory (G) proteins propose that they assume a significant role in the transduction of the signals from the surface of the cell. Molecular Description of the ras p21 Protein ras p21 genetic group does the coding for a gathering that gets firmly to 21kDa (kilo Daltons) proteins having an exceptional proclivity for the guanine nucleotide sequences. On the other hand, the cell proteins having the comparable chemical and biological properties, altogether called the G-proteins, incorporate the G proteins responsible for the regulation of the adenylate cyclase(Spandidos, 2013). Which further involves the regulation of the alpha subcategory of the transducin of the outer bar of the retina. The as of late recognized rho quality proteins, and maybe likewise the prolongation elements. The Guanine proteins consists of the parts within the cell flag transduction, the ras proteins have shared similarities with the cell function within the interceding cell proliferation control. Late advance in the process of cloning and the gene sequencing of the earlier mentioned qualities, the excessive production of the quality items in the Escherichia coli, the engineerin g of the protein(Inoue, et al., 2013). The detailed biochemical portrayal, also, the atomic structure which gets controlled through the high determination the X-beam crystallography, the duo has clarified within the incredible detail of the p21 protein structure including the capacity of the p21 ras proteins. Structure of the Protein The crystalline structure of the protein has a considerable high resolution, among the typical eukaryotic oncogene protein is the c-H-ras. The protein lacks an adaptable of the carboxyl group deposit uncovers p21 protein which comprises of the six beta strands of the protein sheet, further has the four alpha helices, and the nine interfacing circles. About four circles get included in associations with the already bound guanosine diphosphate sequence(Wittinghofer, 2014). The strand sequence which comprises the phosphates, the other strand has a ribose group and the remaining two consists of the bases of guanine. The vast majority of the changing proteins consists of just single amino acids that gets substituted one after another. The organic elements of the staying five circles and other uncovered districts are at present obscure. Be that as it may, one circle relates closely with the binding site for the single cloned counteracting agent and the transformations in the last area don' t modify guanine nucleotide official or guanosine triphosphates movement however they do lessen the changing action of actuated proteins(Wittinghofer, et al., 2013). Relationship of the Ras function as a GTPase Ras p21 assumes an essential part in intracellular flag falls intervening signs from the receptor and the nonreceptor tyrosine kinase to catalyze the cytoplasmic or atomic rections. Mutant types of the Ras p21 contribute in a vital path to the change of an assortment of tumor cells, and in this manner, Ras p21 is named an oncogene. Ras p21, an individual from the G-protein family, motions by performing GTP/GDP nucleotide trade. This can be initiated or hindered by other effector proteins. The structure appeared here is of Ras p21 complexed with a GTP simple (GMPPnP) to imitate the initiated type of the protein(Wittinghofer, et al., 2013). Ras proteins happen to be proto-oncogenes that often get transformed in the human cancers during the process of mutations. They get encoded by the three universally expressed gene including HRAS, KRAS and NRAS. These proteins get the GTPases that capacity as the molecular switches controlling the pathways in charge of the cell proliferation and its survival. The Ras p21 protein assists in the transmission of the signs within the cells commonly referred to as the signal transduction in the cells. The activated Ras aids in the activation of the protein kinase role of the RAF Kinase enzyme(Zhang, et al., 2014). Furthermore, the activated Ras also do activate the serine or the threonine-selective protein kinases in the cell.an example of the serine or the tyrosine or the threonine enzyme is the MEK and the MAPK. The p21 protein regularly gets regulation through the Guanine Nucleotide Exchange Factors (GEFs). The Guanine Nucleotide Exchange Factors helps to enhance the binding activity of the GTP and the role of the GTPase activity in the invigoration of the inherent GTP enzyme that regulates the movement of the protein(Ostrem, et al., 2013). The enzyme GTPase also helps in the turning on conformation and turning it off. The distorted Ras capacity is related to hyperproliferative formative issue and malignancy and in tumors is related to a solitary transformation regularly at codons 12, 13 or 61(Dror, et al., 2015). Change at these monitored destinations favors GTP official and produces constitutive initiation of Ras. Imperatively, all Ras isoforms share succession character in the greater part of the locales in charge of GDP/GTP official, GTPase movement, and effector collaborations proposing useful repetition. Notwithstanding this, it has turned out to be progressively clear that Ras protei ns show isoform-particular capacities. These utilitarian contrasts are no doubt connected with the novel C-terminal hypervariable area (HVR) in each isoform, which is thought to balance the Ras/layer cooperation to indicate particular confinements in organelles and flagging nanoclusters. Binding Characteristics of Ras Protein The ras p21 protein has got the carboxyl terminal domain which facilitates the binding property. Further, the ras [protein has the GTP Binding proteins that perform the critical function in the signaling and the conformation with its binding characteristics. The protein consists of the amino acid sequences. In its structure, three of the amino acids happen to be hydrophobic and always exposed on the surfaces of the protein(Kumar Srivastava, et al., 2016). The hydrophobic amino acids include the A121, A122 and the L171. The hydrophilic amino acids remain buried deep inside the protein. Besides, only two of the total amino acids have got charges, K16 and the D57 amino acids. The two charged amino acids occur deeply inside the protein. The non-charged amino acids remain on the protein surface. The K16 and the D57 amino acids actively participate in the GDP and the GTP binding through the formation of the hydrogen bonds. Although, in the two areas of the ras protein consisting of the ami no acids 116-119 and from 145-147happens to be highly conserved in all the ras molecules and they get themselves involved in the binding to the purine base of the GDP(Stephen, et al., 2014). Hence, the hydrogen bonds get formed in between the N116 and the D119, and the S145 and the A146. The N116 plays a key role in the determination of the conformation of the site for the GDP binding. In the instances in which the Ras protein p21 gets switched on as a result of the incoming cellular signals, the p21 protein would subsequently put on the other proteins, that at the end turns on the genes which get involved in the cell growth, its differentiation and the survival. Usually the mutations in the ras genes leads to the production of the permanently activated p21 proteins(Kumar, et al., 2015). Due to the permanent activation of the Ras proteins would lead to the unintentional and the overactive signaling within the cell, even in the cases where there are no incoming signals. Due to the incoming signals resulting to the cell growth and the division, the overactive Ras signaling results to the ultimate cause of cancer. The three common human genes include the HRas, KRas, and the NRas are the key cancer causative oncogenes in human beings. Conclusion The ras protein p21 belongs to the family of the polypeptides that got commonly found in all the eukaryotic organisms. The protein has a three-dimensional structure that has got folds. As any other protein, it comprises of amino acids which are either polar or non-polar. The amino acids play a significant role in the GDP and the GTP binding to their designated sites. The ras protein gets controlled by the GEfs which promotes the GDP dissociation and stimulates the GTP binding. The protein consists of the amino acid sequences. In its structure, three of the amino acids happen to be hydrophobic and always exposed on the surfaces of the protein. The hydrophobic amino acids include the A121, A122 and the L171. The hydrophilic amino acids remain buried deep inside the protein. In the protein structure, a carboxyl terminal exists which enables the GTP binding capacity. The protein has got the crystalline structure which has a resolution of about 2.7 A. References Dror, R.O., Mildorf, T.J., Hilger, D., Manglik, A., Borhani, D.W., Arlow, D.H., Philippsen, A., Villanueva, N., Yang, Z., Lerch, M.T. and Hubbell, W.L., 2015. Structural basis for nucleotide exchange in heterotrimeric G proteins.Science,348(6241), pp.1361-1365. Inoue, S., Hao, Z., Elia, A.J., Cescon, D., Zhou, L., Silvester, J., Snow, B., Harris, I.S., Sasaki, M., Li, W.Y. and Itsumi, M., 2013. Mule/Huwe1/Arf-BP1 suppresses Ras-driven tumorigenesis by preventing c-Myc/Miz1-mediated down-regulation of p21 and p15.Genes development,27(10), pp.1101-1114. kumar Srivastava, V., Chandra, M., Saito-Nakano, Y., Nozaki, T. and Datta, S., 2016. Crystal structure analysis of wild type and fast hydrolyzing mutant of EhRabX3, a tandem ras superfamily GTPase from entamoeba histolytica.Journal of molecular biology,428(1), pp.41-51. Kumar, A., Najafzadeh, M., Jacob, B.K., Dhawan, A. and Anderson, D., 2015. Zinc oxide nanoparticles affect the expression of p53, Ras p21 and JNKs: an ex vivo/in vitro exposure study in respiratory disease patients.Mutagenesis,30(2), pp.237-245. Ostrem, J.M., Peters, U., Sos, M.L., Wells, J.A. and Shokat, K.M., 2013. K-Ras (G12C) inhibitors allosterically control GTP affinity and effector interactions.Nature,503(7477), pp.548-551.Rhodes, S., Skinner, R.H. and Paget, M.J., 2013. CHARACTERIZATION OF RAS PROTEINS PRODUCED AT HIGH LEVELS IN THE.ras Oncogenes,170, p.141. Spandidos, D. ed., 2013.Ras oncogenes(Vol. 170). Springer Science Business Media. Stephen, A.G., Esposito, D., Bagni, R.K. and McCormick, F., 2014. Dragging ras back in the ring.Cancer cell,25(3), pp.272-281. Wittinghofer, A., 2014.Ras Superfamily Small G Proteins: Biology and Mechanisms 2. Springer International Publishing. Wittinghofer, F., Kabsch, W. and Pai, E.F., 2013. Ute Krengel, Ilme Schlichting, Axel Scheidig, Matthias Frech, Jacob John, Alfred Lautwein.The Superfamily of ras-Related Genes,220, p.183. Wittinghofer, F., Kabsch, W. and Pai, E.F., 2013. Ute Krengel, Ilme Schlichting, Axel Scheidig, Matthias Frech, Jacob John, Alfred Lautwein.The Superfamily of ras-Related Genes,220, p.183. Zhang, S.C., Nouri, K., Amin, E., Taha, M.S., Nakhaeizadeh, H., Nakhaei-Rad, S., Dvorsky, R. and Ahmadian, M.R., 2014. Classical Rho Proteins: Biochemistry of Molecular Switch Function and Regulation. InRas Superfamily Small G Proteins: Biology and Mechanisms 1(pp. 327-340). Springer Vienna.